Regulatory
PpetE
Part:BBa_K273019:Design
Designed by: Karl Brune Group: iGEM09_Uppsala-Sweden (2009-10-18)
PpetE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
-
Source
Synechocystis sp PCC6803
5' -> 3'
Forward primer: CGCgaattcgcggccgcttctagagTCATAGCGGTTGCCCAATC 71,4°C annealing
Reverse primer: GCGctgcagcggccgctactagtaACTTCTTGGCGATTGTATCTATAGGG 69,9°C annealing
References
[http://www.ncbi.nlm.nih.gov/pubmed/2129338 Copper-induced expression, cloning, and regulatory studies of the plastocyanin gene from the cyanobacterium Synechocystis sp. PCC 6803. Plant Mol Biol. 1990 Oct;15(4):633-42]